BLVRA Polyclonal antibody

• Featured Product
• KD/KO Validated

BLVRA Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10775-1-AP

Synonyms

Biliverdin IX alpha reductase, biliverdin reductase A, BLVR, BLVRA, BVR, BVR A, BVRA

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Validation Data Gallery

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  WB analysis using 10775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10775-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10775-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10775-1-AP (BLVRA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10775-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10775-1-AP (BLVRA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of L02 using 10775-1-AP

  Immunofluorescent analysis of L02 cells using 10775-1-AP (BLVRA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, mouse liver tissue, HepG2 cells, HuH-7 cells, PC-3 cells, SH-SY5Y cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10775-1-AP targets BLVRA in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BLVRA fusion protein Ag1166
• Full Name: biliverdin reductase A
• Calculated Molecular Weight: 33.2 kDa
• Observed Molecular Weight: 37-40 kDa, 66 kDa
• GenBank Accession Number: BC008456
• Gene Symbol: BLVRA
• Gene ID (NCBI): 644
• RRID: AB_2065073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BLVRA(Biliverdin reductase A) is also named as BLVR, BVR and belongs to the biliverdin reductase subfamily.It catalyze the conversion of biliverdin to bilirubin in the presence of NADPH or NADH.This protein can exsit as a dimer(PMID:11773068). Defects in BLVRA are the cause of hyperbiliverdinemia (HBLVD).

Publications

Species	Application	Title
human	WB,IHC	Cancer Manag Res; Biliverdin Reductase A (BLVRA) Promotes Colorectal Cancer Cell Progression by Activating the Wnt/β-Catenin Signaling Pathway.; Authors - Haiyan Mao ; KD Validated
human	IF	Arch Biochem Biophys; Biliverdin reductase deficiency triggers an endothelial-to-mesenchymal transition in human endothelial cells.; Authors - Damian Klóska
human	WB	EXCLI J; Induction of cell apoptosis by biliverdin reductase inhibitor in MCF-7 and MDA-MB-468 breast cancer cell lines: Experimental and in silico studies.; Authors - Seyedeh Zahra Shahrokhi