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BAIAP2L1 Polyclonal antibody

BAIAP2L1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 25692-1-AP

Synonyms

BAIAP2L1, IRTKS

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of HeLa using 25692-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of K-562 using 25692-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of SGC-7901 using 25692-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, SGC-7901 cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25692-1-AP is BAIAP2L1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAIAP2L1 fusion protein Ag22499
Full Name BAI1-associated protein 2-like 1
Calculated Molecular Weight 511 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC013888
Gene Symbol BAIAP2L1
Gene ID (NCBI) 55971
RRIDAB_2880197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAIAP2L1, also known as IRTKS, is a 511-amino acid protein containing an IRSp53/MIM homology domain (IMD) that bundles actin filaments and interacts with the small GTPase Rac (PMID: 17430976; 14752106). In addition to the IMD domain, BAIAP2L1 also contains an SH3 domain and WW domain interaction motif. BAIAP2L1 is widely distributed and is an INS receptor substrate. It has a role in actin remodeling and membrane protrusion.

Protocols

Product Specific Protocols
WB protocol for BAIAP2L1 antibody 25692-1-APDownload protocol
IHC protocol for BAIAP2L1 antibody 25692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects both human and mouse versions of the protein.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T and overexpression
BAIAP2L1 Antibody Western Blot, validation (1:1000 dilution) in 293T and overexpression (Cat no:25692-1-AP)