CoraLite® Plus 488-conjugated Annexin A11 Monoclonal antibody

Annexin A11 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A3C4

Cat no : CL488-68089

Synonyms

56 kDa autoantigen, Annexin 11, annexin A11, Annexin XI, ANX11, ANXA11, CAP 50, CAP50

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Validation Data Gallery

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  IF Staining of HeLa using CL488-68089

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 Annexin A11 antibody (CL488-68089, Clone: 1A3C4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-68089

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Annexin A11 (CL488-68089, Clone:1A3C4) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HEK-293T using CL488-68089

  1X10^6 HEK-293T cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Annexin A11 (CL488-68089, Clone:1A3C4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HeLa cells
• Positive FC detected in: HeLa cells, HEK-293T cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68089 targets Annexin A11 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Annexin A11 fusion protein Ag31839
• Full Name: annexin A11
• Calculated Molecular Weight: 56 kDa
• GenBank Accession Number: BC007564
• Gene Symbol: ANXA11
• Gene ID (NCBI): 311
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Annexin A11 (Anxa11) is one member of annexins that are Ca2+-regulated phospholipid-binding proteins. Annexin A11 plays an important role in cell division, differentiation, apoptosis, vesicle trafficking and Ca2+ signaling. Annexin A11 dysregulation is involved in the progression, drug-resistance, recurrence of systemic autoimmune disease and cancer.