CARNS1 Polyclonal antibody

CARNS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25725-1-AP

Synonyms

ATP grasp domain containing 1, ATPGD1, Carnosine synthase 1, CARNS1, KIAA1394

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Validation Data Gallery

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  WB analysis using 25725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25725-1-AP (CARNS1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 25725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 25725-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25725-1-AP (CARNS1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

25725-1-AP targets CARNS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CARNS1 fusion protein Ag22812
• Full Name: ATP-grasp domain containing 1
• Calculated Molecular Weight: 827 aa, 88 kDa
• Observed Molecular Weight: 88-103 kDa
• GenBank Accession Number: BC036557
• Gene Symbol: ATPGD1
• Gene ID (NCBI): 57571
• RRID: AB_2880211
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Transl Stroke Res; Nicotine Exposure Along with Oral Contraceptive Treatment in Female Rats Exacerbates Post-cerebral Ischemic Hypoperfusion Potentially via Altered Histamine Metabolism.; Authors - Nathan d'Adesky
rat	WB	Neurochem Res; Carnosine Reduces Oxidative Stress and Reverses Attenuation of Righting and Postural Reflexes in Rats with Thioacetamide-Induced Liver Failure.; Authors - Krzysztof Milewski