ATP6V1C1 Polyclonal antibody

ATP6V1C1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16054-1-AP

Synonyms

ATP6C, ATP6D, ATP6V1C1, V ATPase subunit C 1, VATC, Vma5



Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, human brain tissue, human testis tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16054-1-AP targets ATP6V1C1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1C1 fusion protein Ag9020
Full Name ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
Calculated Molecular Weight 382 aa, 44 kDa
Observed Molecular Weight 42-44 kDa
GenBank Accession NumberBC010960
Gene Symbol ATP6V1C1
Gene ID (NCBI) 528
RRIDAB_2062501
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ATP6V1C1 antibody 16054-1-APDownload protocol
IHC protocol for ATP6V1C1 antibody 16054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Ther

Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells.

Authors - Benfu Zhong
mouseWB

J Biol Chem

A brain-enriched Drp1 isoform associates with lysosomes, late endosomes and the plasma membrane.

Authors - Kie Itoh
humanWB

Commun Biol

A substrate-trapping strategy to find E3 ubiquitin ligase substrates identifies Parkin and TRIM28 targets.

Authors - Masashi Watanabe
humanWB

Biochem Biophys Res Commun

Lysosome-associated membrane protein-2 deficiency increases the risk of reactive oxygen species-induced ferroptosis in retinal pigment epithelial cells.

Authors - Jong-Jer Lee
humanIHC

Bioengineered

A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma.

Authors - Huimin Shen
humanWB

Autophagy

Inhibition of autophagy and induction of glioblastoma cell death by NEO214, a perillyl alcohol-rolipram conjugate

Authors - Mengting Ou