PARK9/ATP13A2 Polyclonal antibody

PARK9/ATP13A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19141-1-AP

Synonyms

ATP13A2, ATPase type 13A2, HSA9947, KRPPD, PARK9, PARK9/ATP13A2

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Validation Data Gallery

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  WB analysis of mouse brain using 19141-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19141-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19141-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19141-1-AP (PARK9/ATP13A2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19141-1-AP is PARK9/ATP13A2 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARK9/ATP13A2 fusion protein Ag11435
• Full Name: ATPase type 13A2
• Calculated Molecular Weight: 1158 aa, 126 kDa
• Observed Molecular Weight: 125-130 kDa
• GenBank Accession Number: BC030267
• Gene Symbol: ATP13A2
• Gene ID (NCBI): 23400
• RRID: AB_2918064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP13A2 belongs to the P-type superfamily of ATPases that transport inorganic cations and other substrates across cell membranes. It appears to protect cells from manganese and zinc toxicity, possibly by causing cellular efflux and/or lysosomal sequestration; and from iron toxicity, possibly by preserving lysosome integrity against iron-induced lipid peroxidation (21724849)(24334770). However, it potentiates the toxic effects of cadmium and nickel on developing neurites, and of the widely used herbicide paraquat possibly by increasing polyamine uptake. Deficiency is associated with spastic paraplegia and Kufor-Rakeb syndrome, in which there is progressive parkinsonism with dementia.