ASB8 Polyclonal antibody

ASB8 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11735-1-AP

Synonyms

ASB 8, ASB8, PP14212

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Validation Data Gallery

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  WB analysis of human heart using 11735-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11735-1-AP (ASB8 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11735-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11735-1-AP (ASB8 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11735-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11735-1-AP (ASB8 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 11735-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11735-1-AP (ASB8 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11735-1-AP targets ASB8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASB8 fusion protein Ag2361
• Full Name: ankyrin repeat and SOCS box-containing 8
• Calculated Molecular Weight: 288 aa, 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC001321
• Gene Symbol: ASB8
• Gene ID (NCBI): 140461
• RRID: AB_2227489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.