APOBEC3A Polyclonal antibody

APOBEC3A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25084-1-AP

Synonyms

APOBEC3A, ARP3, bK150C2.1, PHO1, Phorbolin 1, PHRBN

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Validation Data Gallery

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  WB analysis using 25084-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25084-1-AP (APOBEC3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HaCaT cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25084-1-AP targets APOBEC3A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOBEC3A fusion protein Ag18845
• Full Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
• Calculated Molecular Weight: 199 aa, 23 kDa
• Observed Molecular Weight: 23 kDa
• GenBank Accession Number: BC126416
• Gene Symbol: APOBEC3A
• Gene ID (NCBI): 200315
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.