ANKS3 Polyclonal antibody

ANKS3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24058-1-AP

Synonyms

ANKS3, KIAA1977

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 24058-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24058-1-AP (ANKS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 24058-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24058-1-AP (ANKS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 24058-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24058-1-AP (ANKS3 Antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, human brain tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 24058-1-AP is ANKS3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKS3 fusion protein Ag21252
• Full Name: ankyrin repeat and sterile alpha motif domain containing 3
• Calculated Molecular Weight: 656 aa, 72 kDa
• Observed Molecular Weight: 72 kDa, 63 kDa
• GenBank Accession Number: BC050387
• Gene Symbol: ANKS3
• Gene ID (NCBI): 124401
• RRID: AB_2879418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKS3 (ankyrin repeat and sterile alpha motif domain containing 3) is a 656 amino acid protein that contain six ANK repeats and one SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. Two isoforms of ANKS3 exist due to alternative splicing events.This antibody may recognize both of them.

Publications

Species	Application	Title
human,mouse	WB,IF,IP,CoIP	PLoS Biol; Bicc1 ribonucleoprotein complexes specifying organ laterality are licensed by ANKS6-induced structural remodeling of associated ANKS3; Authors - Benjamin Rothé