ANKRD57 Polyclonal antibody

ANKRD57 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24033-1-AP

Synonyms

ANKRD57, ankyrin repeat domain 57, C2orf26

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Validation Data Gallery

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  WB analysis using 24033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24033-1-AP (ANKRD57 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24033-1-AP

  IP result of anti-ANKRD57 (IP:24033-1-AP, 3ug; Detection:24033-1-AP 1:1000) with HeLa cells lysate 3000ug.

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  IHC staining of human cerebellum using 24033-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 24033-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 24033-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 24033-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24033-1-AP (ANKRD57 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human cerebellum tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

The immunogen of 24033-1-AP is ANKRD57 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD57 fusion protein Ag21238
• Full Name: ankyrin repeat domain 57
• Calculated Molecular Weight: 525 aa, 56 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC036652
• Gene Symbol: ANKRD57
• Gene ID (NCBI): 65124
• RRID: AB_2879412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Immunol; A DNA-Methylation-Driven Genes Based Prognostic Signature Reveals Immune Microenvironment in Pancreatic Cancer.; Authors - Mingjia Xiao