ANKRD54 Polyclonal antibody

ANKRD54 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25738-1-AP

Synonyms

ANKRD54, ankyrin repeat domain 54

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Validation Data Gallery

• 

  WB analysis of mouse testis using 25738-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 25738-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25738-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25738-1-AP (ANKRD54 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 25738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 25738-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25738-1-AP (ANKRD54 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 25738-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25738-1-AP (ANKRD54 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 25738-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25738-1-AP (ANKRD54 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse testis tissue, K-562 cells, PC-3 cells
• Positive IHC detected in: mouse testis tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25738-1-AP is ANKRD54 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD54 fusion protein Ag22759
• Full Name: ankyrin repeat domain 54
• Calculated Molecular Weight: 300 aa, 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC066909
• Gene Symbol: ANKRD54
• Gene ID (NCBI): 129138
• RRID: AB_2880218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.