ANKRD53 Polyclonal antibody

ANKRD53 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 24283-1-AP

Synonyms

ANKRD53, ankyrin repeat domain 53

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Validation Data Gallery

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  WB analysis of HeLa using 24283-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24283-1-AP (ANKRD53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24283-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24283-1-AP (ANKRD53 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 24283-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24283-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24283-1-AP (ANKRD53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 24283-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 24283-1-AP (ANKRD53 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human kidney tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24283-1-AP is ANKRD53 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD53 fusion protein Ag15030
• Full Name: ankyrin repeat domain 53
• Calculated Molecular Weight: 530 aa, 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC035234
• Gene Symbol: ANKRD53
• Gene ID (NCBI): 79998
• RRID: AB_2879470
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANKRD53 also termed as ankyrin repeat domain 53 is a 533 amino-acid protein,which contains three ANK repeats and belongs to ankyrin family. The function of ANKRD53 is still non-known and need to be further studied. Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network.