ANKRD50 Polyclonal antibody

ANKRD50 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 24032-1-AP

Synonyms

ANKRD50, ankyrin repeat domain 50, KIAA1223

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Validation Data Gallery

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  WB analysis of HEK-293 using 24032-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24032-1-AP (ANKRD50 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24032-1-AP

  IP result of anti-ANKRD50 (IP:24032-1-AP, 5ug; Detection:24032-1-AP 1:300) with HEK-293 cells lysate 4000ug.

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  IF Staining of HEK-293 using 24032-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24032-1-AP (ANKRD50 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24032-1-AP is ANKRD50 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANKRD50 fusion protein Ag21237
• Full Name: ankyrin repeat domain 50
• Calculated Molecular Weight: 1429 aa, 156 kDa
• Observed Molecular Weight: 137-156 kDa
• GenBank Accession Number: BC024725
• Gene Symbol: ANKRD50
• Gene ID (NCBI): 57182
• RRID: AB_2879411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.