AKR1C3 Polyclonal antibody

AKR1C3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11194-1-AP

Synonyms

17 beta HSD 5, 3 alpha HSD type 2, 3 alpha HSD type II, brain, AKR1C3, DD 3, DD3, DDX, Dihydrodiol dehydrogenase 3, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, Indanol dehydrogenase, KIAA0119, PGFS, Prostaglandin F synthase

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Validation Data Gallery

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  WB analysis using 11194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11194-1-AP

  IP result of anti-AKR1C3 (IP:11194-1-AP, 4ug; Detection:11194-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human prostate cancer using 11194-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11194-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, Jurkat cells, K-562 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

11194-1-AP targets AKR1C3 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AKR1C3 fusion protein Ag1674
• Full Name: aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• Calculated Molecular Weight: 323 aa, 37 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC019230
• Gene Symbol: AKR1C3
• Gene ID (NCBI): 8644
• RRID: AB_2224414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism. The rat kidney possesses a dimeric form of 75 kDa(PMID:18574251).

Publications

Species	Application	Title
human	WB	Mol Cancer Ther; AKR1C3 promotes AR-V7 protein stabilization and confers resistance to AR-targeted therapies in advanced prostate cancer.; Authors - Chengfei Liu
human	WB,IHC	World J Gastroenterol; Prostaglandin F2α synthase promotes oxaliplatin resistance in colorectal cancer through prostaglandin F2α-dependent and F2α-independent mechanism; Authors - Yi-Jun Wang
human	WB	Int J Mol Med; 15-Deoxy-Δ(12,14)-prostaglandin J(2) induces growth inhibition, cell cycle arrest and apoptosis in human endometrial cancer cell lines.; Authors - Li Haili H
mouse,rat	WB	Neurosci Lett; Mangiferin alleviates 6-OHDA-induced Parkinson's disease by inhibiting AKR1C3 to activate Wnt signaling pathway; Authors - Wanran Huang
human	WB	Eur J Med Chem; Development of highly potent and specific AKR1C3 inhibitors to restore the chemosensitivity of drug-resistant breast cancer; Authors - Yang Liu
	WB	bioRxiv; Differential regulation of Retinoic Acid Metabolism in Fanconi Anemia; Authors - Justin L Blaize