AHCYL2 Monoclonal antibody

AHCYL2 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1D1F6

Cat no : 67484-1-Ig

Synonyms

AdoHcyase 3, AHCYL2, FLJ21719, KIAA0828

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Validation Data Gallery

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  WB analysis of A549 using 67484-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 67484-1-Ig (AHCYL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67484-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67484-1-Ig (AHCYL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67484-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67484-1-Ig (AHCYL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 67484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67484-1-Ig (AHCYL2 antibody) at dilution of 1:600 (under 10x lens)..

• 

  IHC staining of human stomach using 67484-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 67484-1-Ig (AHCYL2 antibody) at dilution of 1:600 (under 40x lens)..

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells, HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67484-1-Ig targets AHCYL2 in WB, IHC,ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: AHCYL2 fusion protein Ag19614
• Full Name: S-adenosylhomocysteine hydrolase-like 2
• Calculated Molecular Weight: 611 aa, 67 kDa
• Observed Molecular Weight: 67 and 57 kDa
• GenBank Accession Number: BC024325
• Gene Symbol: AHCYL2
• Gene ID (NCBI): 23382
• RRID: AB_2882711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.