AGMAT Polyclonal antibody

AGMAT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21899-1-AP

Synonyms

AGMAT, Agmatinase, Agmatinase mitochondrial, Agmatine ureohydrolase, AUH

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Validation Data Gallery

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  WB analysis using 21899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21899-1-AP (AGMAT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21899-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21899-1-AP (AGMAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21899-1-AP targets AGMAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGMAT fusion protein Ag13609
• Full Name: agmatine ureohydrolase (agmatinase)
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC005090
• Gene Symbol: AGMAT
• Gene ID (NCBI): 79814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGMAT is a metallohydrolase and belongs to the arginase family. AGMAT plays an important role in amino acid metabolism and can hydrolyze agmatine into urea and putrescine. AGMAT has been shown to play an important role in the development of tumors, including colorectal cancer, lung adenocarcinoma, and pancreatic adenocarcinoma(PMID: 36680755; PMID: 31699997; PMID: 35837051 ).