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Phospho-Marcksl1 Polyclonal antibody

Phospho-Marcksl1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (3)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10018-3-AP

Synonyms

MARCKS, MARCKS-like 1, PKCSL, Myristoylated alanine-rich C-kinase substrate, Marcksl1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-Marcksl1 Polyclonal antibody (10018-3-AP)

    WB analysis using 10018-3-AP

    Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10018-3-AP (Phospho-Marcksl1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

Tested Applications

Positive WB detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 5 publications below

Product Information

10018-3-AP targets Phospho-Marcksl1 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivityhuman, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myristoylated alanine rich protein kinase C substrate
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC046601
Gene Symbol Marcks
Gene ID (NCBI) 17118
RRIDAB_2297345
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated. Because of the sequence similarity, this antibody can recognize phospho-Marcksl1 and phospho-Marcks.

Protocols

Product Specific Protocols
WB protocol for Phospho-Marcksl1 antibody 10018-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB

Proc Natl Acad Sci U S A

MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.

Authors - Jin Benjamin Y BY
View Article
ratWB

J Neurosci

NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia.

Authors - Peng Y-J YJ
View Article
ratWB

J Physiol

5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase.

Authors - Peng Ying-Jie YJ
View Article
ratWB

Am J Physiol Cell Physiol

Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability.

Authors - Tinsley John H JH
View Article
humanWB

Open Virol J

HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.

Authors - Fiala Milan M
View Article