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  • Featured Product
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CISD1 Polyclonal antibody

CISD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16006-1-AP

Synonyms

MDS029, EC:2.6.1.3, Cysteine transaminase CISD1, CDGSH iron-sulfur domain-containing protein 1, CDGSH iron sulfur domain 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CISD1 Polyclonal antibody (16006-1-AP)

    WB analysis using 16006-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16006-1-AP (CISD1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CISD1 Polyclonal antibody (16006-1-AP)

    IP experiment of HepG2 using 16006-1-AP

    IP result of anti-CISD1 (IP:16006-1-AP, 3ug; Detection:16006-1-AP 1:2000) with HepG2 cells lysate 600ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CISD1 Polyclonal antibody (16006-1-AP)

    IHC staining of human pancreas cancer using 16006-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16006-1-AP (CISD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CISD1 Polyclonal antibody (16006-1-AP)

    IF Staining of HepG2 using 16006-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD1 antibody (16006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse skeletal muscle tissue, rat kidney tissue, rat skeletal muscle tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16006-1-AP targets CISD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CISD1 fusion protein Ag8680
Full Name CDGSH iron sulfur domain 1
Calculated Molecular Weight 108 aa, 12 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC007043
Gene Symbol CISD1
Gene ID (NCBI) 55847
RRIDAB_2080268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.

Protocols

Product Specific Protocols
WB protocol for CISD1 antibody 16006-1-APDownload protocol
IHC protocol for CISD1 antibody 16006-1-APDownload protocol
IF protocol for CISD1 antibody 16006-1-APDownload protocol
IP protocol for CISD1 antibody 16006-1-APDownload protocol
FC protocol for CISD1 antibody 16006-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Mechanism and regulation of the Lys6-selective deubiquitinase USP30.

Authors - Malte Gersch
View Article
mouseWB

Cell Metab

Basal Mitophagy Occurs Independently of PINK1 in Mouse Tissues of High Metabolic Demand.

Authors - Thomas G McWilliams
View Article
humanWB

Mol Cell

Quantitative proteomics reveal a feedforward mechanism for mitochondrial PARKIN translocation and ubiquitin chain synthesis.

Authors - Alban Ordureau
View Article
humanWB

Mol Cell

Dynamics of PARKIN-Dependent Mitochondrial Ubiquitylation in Induced Neurons and Model Systems Revealed by Digital Snapshot Proteomics.

Authors - Alban Ordureau
View Article

Mol Cell

Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling.

Authors - Alban Ordureau
View Article
humanWB

Sci Adv

AMPK/ULK1-mediated phosphorylation of Parkin ACT domain mediates an early step in mitophagy.

Authors - Chien-Min Hung
View Article