GFP tag Monoclonal antibody

Zebrafish embryos expressing cytosolic GFP

• Applications: Western Blot
• Cell Tissue Type: Zebrafish embryos

Detected EGFP-tagged constructs expressed in HEK293T cells at similar strength to other antibodies however with slightly more background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: HEK293T

Works great. No problem. Love proteintech.

• Applications: Western Blot
• Primary Antibody Dilution: 1:25000
• Cell Tissue Type: Bacteria

Good signal, antibody can be reused. Overexpressed GFP-tagged proteins (and GFP alone) were pulled down using GFP-Trap beads and WB was done on lysates and IP samples. Antibody was diluted in 4% milk (and could be reused several times).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human / HeLa cells

It works very well

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: Murine Breast cancer

works great for Western blot!

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: HEK293T

Of the 3 antibodies tested for GFP this one gave the cleanest and strongest signal by western blot. When I saw it on sale, I bought 8!

• Applications: Western Blot
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: Hap1, N2a (mouse neuroblastoma)

SDS-PAGE: 15 ug/ul RIPA protein lysate, 4-12% Bis-Tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) for 2h at 80V, 4C.Blocking: SEA Block Blocking Buffer 1h, room T.Primary Ab: O/N incubation at 4C, 1:5000.Secondary Ab: IRDye 800CW Goat anti-Mouse, 1:15000.Lines of WB image: 1 – protein ladder, 2 – HEK293 whole cell lysate, negative transfection, 3 – whole cell lysate of cells transfected with eGFP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293

HEK293T and HEK293T stably transfected with pAcGFP plasmid. 10ug total protein loaded per well. Membrane blocked 1 hour in 5% BSA prior to anti-GFP (1:2000) o/n at 4 degrees. Goat anti-mouse HRP secondary (1:10,000) used.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

Adenovirus-GFP infected cardiomyocytes stained with GFP antibody, signal is strong and effective

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: cardiomyocyte culture

GOOD

• Applications: Immunofluorescence,
• Cell Tissue Type: HEART

Worked for western blot with GFP-LC3 transfected cardiomyocytes

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cardiomyocytes

Works well for Westerns.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: HEK293T

Good antibody for Western Blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

Worked well for WB

• Applications: Western Blot,
• Primary Antibody Dilution: 5000
• Cell Tissue Type: HEKs

Easily detects recombinant GFP protein via western blot with very little background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500 to 1:5000
• Cell Tissue Type: Recombinant GFP protein

Used this to detect tranduce efficiency of the AAV-GFP virus. with 5% non-fat milk, there are two bands around 20-30kd, not sure which one is correct. And the antibody is relative weak, has to use 1:500 dilution.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse heart

The GFP antibody showed great sensitivity for WB and it was easily detecable. But it was insteresting that when the SDS PAGE gel separating gel concentration reeached to 12%, you can see two bands at ~30kDa and ~20kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: 293T

This is a good antibody for detecting GFP tag on Western blot, using 1:1000 dilution. The price is unbeatable, worth each penny.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: GFP-tagged protein expressed in human cell lines

antibody used in western blot against egfp labelled protien and clear band was detected.

• Applications: Western Blot,
• Primary Antibody Dilution: 1 to 4000