GFP tag Antibody 37 Publications

Mouse Monoclonal| Catalog number: 66002-1-Ig |CloneNo.: 1E10H7

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Freight/Packing

Size: 09µ/gram

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Species specificity:
Recombinant protein, human, Tag

Positive WB detected in:
Recombinant Protein

Positive IP detected in:
Transfected HEK-293 cells

Positive IF detected in:
Transfected HEK-293 cells

Recommended dilution:
WB : 1:2000-1:20000
IP : 1:1000-1:10000
IF : 1:20-1:200

Product Information


Source:
Mouse

Purification method:
Protein A purification

Isotype:
IgG2a

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.

Immunogen Information


Full name:
GFP tag

Calculated molecular weight:
26 kDa

Observed molecular weight:

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
CFP, eGFP, eYFP, GFP, GFP tag, YFP
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYPF tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.


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