GFP tag AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IP, IF|
|Cited applications||IF, IHC, WB|
|Species specificity||Recombinant protetin, Aequorea victoria, Human; other species not tested.|
|Positive WB detected in||recombinant protein|
|Positive IP detected in||Transfected HEK-293 cells|
|Positive IF detected in||Transfected HEK-293 cells|
|Recommended dilution||WB: 1:1000-1:10000|
IP = Immunoprecipitation
- Western blot of eGFP-tagged fusion protein with anti-eGFP-tag (50430-2-AP) at various dilutions.
- IP Result of anti-GFP tag (IP:50430-2-AP, 4ug; Detection:50430-2-AP 1:5000) with Transfected HEK-293 cells lysate 300ug.
- Immunofluorescent analysis of Transfected HEK-293 cells using 50430-2-AP( GFP tag Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||GFP tag fusion protein ag2128||Full name||GFP tag|
|AVU73901||Gene ID (NCBI)|
|Gene symbol||Synonyms||CFP, eGFP, eYFP, GFP, GFP tag, YFP|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.