Dynamitin (1-401 AA) Monoclonal antibody
Dynamitin (1-401 AA) Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2B9A5
Cat no : 60257-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
The immunogen of 60257-1-Ig is Dynamitin (1-401 AA) Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Dynamitin (1-401 AA) fusion protein Ag1902 |
| Full Name | dynactin 2 (p50) |
| Calculated Molecular Weight | 401 aa, 44 kDa |
| Observed Molecular Weight | 52+150 kDa |
| GenBank Accession Number | BC014083 |
| Gene Symbol | Dynamitin |
| Gene ID (NCBI) | 10540 |
| RRID | AB_2881378 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig | Download protocol |
| IHC protocol for Dynamitin (1-401 AA) antibody 60257-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Biol Knockdown of Dynamitin in testes significantly decreased male fertility in Drosophila melanogaster. |
