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domain-II-of-FIZ1 Polyclonal antibody

domain-II-of-FIZ1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15827-1-AP

Synonyms

domain I of FIZ 1, domain II of FIZ1, FIZ1, FLT3 interacting zinc finger 1, Zinc finger protein 798, ZNF798

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using domain-II-of-FIZ1 Polyclonal antibody (15827-1-AP)

    WB analysis of human kidney using 15827-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15827-1-AP (domain-II-of-FIZ1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using domain-II-of-FIZ1 Polyclonal antibody (15827-1-AP)

    WB analysis of human kidney using 15827-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15827-1-AP (domain-II-of-FIZ1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using domain-II-of-FIZ1 Polyclonal antibody (15827-1-AP)

    IHC staining of human kidney using 15827-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15827-1-AP (domain-II-of-FIZ1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using domain-II-of-FIZ1 Polyclonal antibody (15827-1-AP)

    IHC staining of human kidney using 15827-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15827-1-AP (domain-II-of-FIZ1 Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inhuman kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15827-1-AP targets domain-II-of-FIZ1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name FLT3-interacting zinc finger 1
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberNM_032836
Gene Symbol FIZ1
Gene ID (NCBI) 84922
RRIDAB_2102545
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FIZ1, also named as ZNF798, may be a transcriptional repressor of NRL function in photoreceptors. FIZ1 does not repress CRX-mediated transactivation.

Protocols

Product Specific Protocols
WB protocol for domain-II-of-FIZ1 antibody 15827-1-APDownload protocol
IHC protocol for domain-II-of-FIZ1 antibody 15827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols