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ZXDC Polyclonal antibody

ZXDC Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20530-1-AP

Synonyms

Zinc finger protein ZXDC, ZXD family zinc finger C, ZXD like zinc finger protein, ZXDC, ZXDL

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human kidney tissue using ZXDC Polyclonal antibody (20530-1-AP)

    WB analysis of human kidney using 20530-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZXDC Polyclonal antibody (20530-1-AP)

    WB analysis of HEK-293 using 20530-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ZXDC Polyclonal antibody (20530-1-AP)

    WB analysis of mouse kidney using 20530-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using ZXDC Polyclonal antibody (20530-1-AP)

    WB analysis of A431 using 20530-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20530-1-AP (ZXDC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZXDC Polyclonal antibody (20530-1-AP)

    IF Staining of HeLa using 20530-1-AP

    Immunofluorescent analysis of Hela cells, using ZXDC antibody 20530-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman kidney tissue, A431 cells, HEK-293 cells, mouse kidney tissue
Positive IF/ICC detected inHela cells

ZXDC has low expression in most cells and tissues

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20530-1-AP is ZXDC Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZXDC fusion protein Ag14392
Full Name ZXD family zinc finger C
Calculated Molecular Weight 858 aa, 90 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC002940
Gene Symbol ZXDC
Gene ID (NCBI) 79364
RRIDAB_10694823
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZXDC antibody 20530-1-APDownload protocol
IF protocol for ZXDC antibody 20530-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Cell-fate transition and determination analysis of mouse male germ cells throughout development.

Authors - Jiexiang Zhao
View Article
humanWB,IHC

iScience

ZXDC enhances cervical cancer metastasis through IGF2BP3-mediated activation of RhoA/ROCK signaling

Authors - Yifang Mao
View Article
human,mouseWB

Cancer Cell

The crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia

Authors - Mingyang Liu
View Article