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ZWINT Polyclonal antibody

ZWINT Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12282-2-AP

Synonyms

Zwint-1, ZWINT1, Zwint 1, ZW10-interacting protein 1, KNTC2AP

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZWINT Polyclonal antibody (12282-2-AP)

    WB analysis of HepG2 using 12282-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12282-2-AP (ZWINT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZWINT Polyclonal antibody (12282-2-AP)

    IF Staining of HepG2 using 12282-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZWINT antibody (12282-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using ZWINT Polyclonal antibody (12282-2-AP)

    FC experiment of HeLa using 12282-2-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZWINT (12282-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12282-2-AP targets ZWINT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZWINT fusion protein Ag2933
Full Name ZW10 interactor
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC020979
Gene Symbol ZWINT
Gene ID (NCBI) 11130
RRIDAB_2221166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZWINT antibody 12282-2-APDownload protocol
IF protocol for ZWINT antibody 12282-2-APDownload protocol
FC protocol for ZWINT antibody 12282-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols