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ZWILCH Polyclonal antibody

ZWILCH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14281-1-AP

Synonyms

Protein zwilch homolog, KNTC1AP, hZwilch

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ZWILCH Polyclonal antibody (14281-1-AP)

    WB analysis of PC-3 using 14281-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14281-1-AP (ZWILCH Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ZWILCH Polyclonal antibody (14281-1-AP)

    IHC staining of human colon cancer using 14281-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14281-1-AP (ZWILCH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14281-1-AP targets ZWILCH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZWILCH fusion protein Ag5454
Full Name Zwilch, kinetochore associated, homolog (Drosophila)
Calculated Molecular Weight 67 kDa
Observed Molecular Weight ~60 kDa
GenBank Accession NumberBC036900
Gene Symbol ZWILCH
Gene ID (NCBI) 55055
RRIDAB_2878040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZWILCH is a new component of the ZW10/ROD complex. The complex is a key player in the spindle checkpoint. ZWILCH has two isoforms with MW 67 kDa and 54 kDa.

Protocols

Product Specific Protocols
WB protocol for ZWILCH antibody 14281-1-APDownload protocol
IHC protocol for ZWILCH antibody 14281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nucleic Acids Res

ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.

Authors - Fengjie Yuan
View Article
humanIHC

Biomedicines

The Enhanced Expression of ZWILCH Predicts Poor Survival of Adrenocortical Carcinoma Patients

Authors - Małgorzata Blatkiewicz
View Article