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ZSCAN20 Polyclonal antibody

ZSCAN20 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20855-1-AP

Synonyms

KOX29, ZFP 31, Zinc finger protein 31, Zinc finger protein 360, Zinc finger protein KOX29, ZNF31, ZNF360, ZSCAN20

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZSCAN20 Polyclonal antibody (20855-1-AP)

    WB analysis of HepG2 using 20855-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZSCAN20 Polyclonal antibody (20855-1-AP)

    WB analysis of Jurkat using 20855-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZSCAN20 Polyclonal antibody (20855-1-AP)

    WB analysis of Jurkat using 20855-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20855-1-AP (ZSCAN20 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ZSCAN20 Polyclonal antibody (20855-1-AP)

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ZSCAN20 Polyclonal antibody (20855-1-AP)

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:200.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ZSCAN20 Polyclonal antibody (20855-1-AP)

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:200.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ZSCAN20 Polyclonal antibody (20855-1-AP)

    IHC staining of human small intestine using 20855-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 20855-1-AP (ZSCAN20 Antibody) at dilution of 1:400.

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20855-1-AP is ZSCAN20 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZSCAN20 fusion protein Ag14798
Full Name zinc finger and SCAN domain containing 20
Calculated Molecular Weight 1043 aa, 118 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC008827
Gene Symbol ZSCAN20
Gene ID (NCBI) 7579
RRIDAB_2878753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZSCAN20 antibody 20855-1-APDownload protocol
IHC protocol for ZSCAN20 antibody 20855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols