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ZPBP Polyclonal antibody

ZPBP Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 15835-1-AP

Synonyms

SP38, zona pellucida binding protein, ZPBP, ZPBP1

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse testis tissue using ZPBP Polyclonal antibody (15835-1-AP)

    IP experiment of mouse testis using 15835-1-AP

    IP result of anti-ZPBP (IP:15835-1-AP, 3ug; Detection:15835-1-AP 1:300) with mouse testis tissue lysate 2800ug.

Tested Applications

Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15835-1-AP targets ZPBP in IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZPBP fusion protein Ag8585
Full Name zona pellucida binding protein
Calculated Molecular Weight 351 aa, 40 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC005223
Gene Symbol ZPBP
Gene ID (NCBI) 11055
RRIDAB_2878189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZPBP (also known as ZPBP1), named for its binding to the oocyte zona pellucida, is a spermatid and spermatozoon protein that localizes to the acrosome. ZPBP may be implicated in gamete interaction during fertilization. It has been reported that absence of ZPBP1 prevents acrosome compaction and leads to acrosome fragmentation and sterility in male mice. (PMID: 17664285; 19204925)

Protocols

Product Specific Protocols
IP protocol for ZPBP antibody 15835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols