ZNHIT1 Polyclonal antibody

ZNHIT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16595-1-AP

Synonyms

Zinc finger HIT domain-containing protein 1, p18 Hamlet, Cyclin-G1-binding protein 1, Cyclin G1 binding protein 1, CGBP1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, PC-12 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16595-1-AP targets ZNHIT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNHIT1 fusion protein Ag9741
Full Name zinc finger, HIT type 1
Calculated Molecular Weight 154 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC017333
Gene Symbol ZNHIT1
Gene ID (NCBI) 10467
RRIDAB_2220382
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNHIT1(Zinc finger HIT domain containing protein 1), aslo named CGBP1 (Cyclin G1 binding protein 1), is a subunit of the SNF-2 related CBP activator protein (SRCAP) complex, which can remodel chromatin by incorporating the histone variant H2A.Z into nucleosomes(PMID: 15647280). ZNHIT1 also can bind to myogenin promoter and mediate H2A.Z incorporation for its expression(PMID: 20473270). ZNHIT1 overexpression induces apoptosis and represses proliferation and invasion of breast cancer cells(PMID: 30928405).

Protocols

Product Specific Protocols
WB protocol for ZNHIT1 antibody 16595-1-APDownload protocol
IHC protocol for ZNHIT1 antibody 16595-1-APDownload protocol
IF protocol for ZNHIT1 antibody 16595-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

ANP32E is a histone chaperone that removes H2A.Z from chromatin.

Authors - Arnaud Obri
WB

Cell Discov

Structural insights into histone exchange by human SRCAP complex

Authors - Jiali Yu