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ZNF839 Polyclonal antibody

ZNF839 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20539-1-AP

Synonyms

Zinc finger protein 839, Renal carcinoma antigen NY-REN-50, C14orf131

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF839 Polyclonal antibody (20539-1-AP)

    WB analysis of HeLa using 20539-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20539-1-AP (ZNF839 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human small intestine tissue using ZNF839 Polyclonal antibody (20539-1-AP)

    WB analysis of human small intestine using 20539-1-AP

    human small intestine tissue were subjected to SDS PAGE followed by western blot with 20539-1-AP (ZNF839 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ZNF839 Polyclonal antibody (20539-1-AP)

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ZNF839 Polyclonal antibody (20539-1-AP)

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF839 Polyclonal antibody (20539-1-AP)

    IF Staining of HepG2 using 20539-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF839 antibody 20539-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF839 Polyclonal antibody (20539-1-AP)

    IF Staining of HeLa using 20539-1-AP

    Immunofluorescent analysis of Hela cells, using ZNF839 antibody 20539-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, human small intestine tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20539-1-AP targets ZNF839 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF839 fusion protein Ag14573
Full Name zinc finger protein 839
Calculated Molecular Weight 927 aa, 99 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC006222
Gene Symbol ZNF839
Gene ID (NCBI) 55778
RRIDAB_10695759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF839 antibody 20539-1-APDownload protocol
IHC protocol for ZNF839 antibody 20539-1-APDownload protocol
IF protocol for ZNF839 antibody 20539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols