- Featured Product
- KD/KO Validated
ZNF746 Polyclonal antibody
ZNF746 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 24543-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HEK-293T cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
24543-1-AP targets ZNF746 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ZNF746 fusion protein Ag21566 |
| Full Name | zinc finger protein 746 |
| Calculated Molecular Weight | 644 aa, 69 kDa |
| Observed Molecular Weight | 85-95 kDa |
| GenBank Accession Number | BC068505 |
| Gene Symbol | ZNF746 |
| Gene ID (NCBI) | 155061 |
| RRID | AB_2879598 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN (PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF746 antibody 24543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease.
| ||
Sci Adv Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease. | ||
Brain Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease. | ||
Brain Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration. | ||
Front Aging Neurosci Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice | ||
Biochem Biophys Res Commun ZNF746/PARIS promotes the occurrence of hepatocellular carcinoma.
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