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ZNF649 Polyclonal antibody

ZNF649 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25797-1-AP

Synonyms

zinc finger protein 649, ZNF649

Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using ZNF649 Polyclonal antibody (25797-1-AP)

    WB analysis of HL-60 using 25797-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25797-1-AP (ZNF649 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ZNF649 Polyclonal antibody (25797-1-AP)

    WB analysis of mouse heart using 25797-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25797-1-AP (ZNF649 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHL-60 cells, mouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25797-1-AP is ZNF649 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF649 fusion protein Ag22700
Full Name zinc finger protein 649
Calculated Molecular Weight 505 aa, 58 kDa
Observed Molecular Weight 50-57 kDa
GenBank Accession NumberBC005368
Gene Symbol ZNF649
Gene ID (NCBI) 65251
RRIDAB_2880241
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF649, also named as Zinc finger protein 649, is a 505 amino acid protein, which contains The one KRAB domain and belongs to the krueppel C2H2-type zinc-finger protein family.ZNF649 is highly expressed in heart, skeletal muscle, and brain and lower expression in liver, lung, kidney, pancreas and placenta. ZNF649 as a regulator of transcriptional factor complexes may suppress SRE and AP-1 transcription activities by growth factor signaling pathways.

Protocols

Product Specific Protocols
WB protocol for ZNF649 antibody 25797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols