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ZNF639 Polyclonal antibody

ZNF639 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25817-1-AP

Synonyms

6230400O18Rik, ANC 2H01, ANC_2H01, ZASC1, zinc finger protein 639, Zinc finger protein ANC_2H01, Zinc finger protein ZASC1, ZNF639

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF639 Polyclonal antibody (25817-1-AP)

    WB analysis of HeLa using 25817-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25817-1-AP (ZNF639 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZNF639 Polyclonal antibody (25817-1-AP)

    IP experiment of HeLa using 25817-1-AP

    IP result of anti-ZNF639 (IP:25817-1-AP, 4ug; Detection:25817-1-AP 1:300) with HeLa cells lysate 3960ug.

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25817-1-AP is ZNF639 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF639 fusion protein Ag19854
Full Name zinc finger protein 639
Calculated Molecular Weight 485 aa, 56 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC026181
Gene Symbol ZNF639
Gene ID (NCBI) 51193
RRIDAB_2880251
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF639, also named as ZASC1, is a 485 amino acid protein, which locates in the nucleus and contains 8 C2H2-type zinc fingers and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF639 binds DNA and may function as a transcriptional repressor.

Protocols

Product Specific Protocols
WB protocol for ZNF639 antibody 25817-1-APDownload protocol
IP protocol for ZNF639 antibody 25817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols