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ZNF622 Polyclonal antibody

ZNF622 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20804-1-AP

Synonyms

Zinc finger like protein 9, zinc finger protein 622, ZNF622, ZPR9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF622 Polyclonal antibody (20804-1-AP)

    WB analysis of HeLa using 20804-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20804-1-AP (ZNF622 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZNF622 Polyclonal antibody (20804-1-AP)

    WB analysis of HEK-293 using 20804-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20804-1-AP (ZNF622 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using ZNF622 Polyclonal antibody (20804-1-AP)

    WB analysis of human brain using 20804-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20804-1-AP (ZNF622 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ZNF622 Polyclonal antibody (20804-1-AP)

    WB analysis of mouse lung using 20804-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20804-1-AP (ZNF622 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ZNF622 Polyclonal antibody (20804-1-AP)

    IHC staining of human hepatocirrhosis using 20804-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20804-1-AP (ZNF622 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using ZNF622 Polyclonal antibody (20804-1-AP)

    IHC staining of human hepatocirrhosis using 20804-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20804-1-AP (ZNF622 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using ZNF622 Polyclonal antibody (20804-1-AP)

    IHC staining of human lung using 20804-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20804-1-AP (ZNF622 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using ZNF622 Polyclonal antibody (20804-1-AP)

    IHC staining of human lung using 20804-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 20804-1-AP (ZNF622 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF622 Polyclonal antibody (20804-1-AP)

    IF Staining of HeLa using 20804-1-AP

    Immunofluorescent analysis of Hela cells, using ZNF622 antibody 20804-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF622 Polyclonal antibody (20804-1-AP)

    IF Staining of HepG2 using 20804-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF622 antibody 20804-1-AP at 1:25 dilution and FITC- labeled donkey anti-Rabbit IgG (green).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human brain tissue, mouse lung tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20804-1-AP is ZNF622 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF622 fusion protein Ag14741
Full Name zinc finger protein 622
Calculated Molecular Weight 477 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC008752
Gene Symbol ZNF622
Gene ID (NCBI) 90441
RRIDAB_10693628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF622 antibody 20804-1-APDownload protocol
IHC protocol for ZNF622 antibody 20804-1-APDownload protocol
IF protocol for ZNF622 antibody 20804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols