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ZNF597 Polyclonal antibody

ZNF597 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20922-1-AP

Synonyms

zinc finger protein 597, ZNF597

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ZNF597 Polyclonal antibody (20922-1-AP)

    WB analysis of Jurkat using 20922-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20922-1-AP (ZNF597 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF597 Polyclonal antibody (20922-1-AP)

    WB analysis of HepG2 using 20922-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20922-1-AP (ZNF597 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF597 Polyclonal antibody (20922-1-AP)

    WB analysis of HeLa using 20922-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20922-1-AP (ZNF597 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF597 Polyclonal antibody (20922-1-AP)

    IHC staining of human brain using 20922-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20922-1-AP (ZNF597 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using ZNF597 Polyclonal antibody (20922-1-AP)

    IHC staining of human brain using 20922-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20922-1-AP (ZNF597 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20922-1-AP is ZNF597 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF597 fusion protein Ag15183
Full Name zinc finger protein 597
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC029899
Gene Symbol ZNF597
Gene ID (NCBI) 146434
RRIDAB_10859828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF597 antibody 20922-1-APDownload protocol
IHC protocol for ZNF597 antibody 20922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols