ZNF571 Polyclonal antibody
ZNF571 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 25302-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HeLa cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 25302-1-AP is ZNF571 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ZNF571 fusion protein Ag18151 |
Full Name | zinc finger protein 571 |
Calculated Molecular Weight | 609 aa, 71 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC114479 |
Gene Symbol | ZNF571 |
Gene ID (NCBI) | 51276 |
RRID | AB_2880019 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for ZNF571 antibody 25302-1-AP | Download protocol |
IHC protocol for ZNF571 antibody 25302-1-AP | Download protocol |
IP protocol for ZNF571 antibody 25302-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Daniel (Verified Customer) (10-26-2022) | This antibody cross-reacts with other protein and produces several non-specific bands. The band of interest is usually sandwiched between two non-specific bands.
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