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  • Featured Product
  • KD/KO Validated

ZNF451 Polyclonal antibody

ZNF451 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25228-1-AP

Synonyms

KIAA1702, KIAA0576, EC:2.3.2.-, E3 SUMO-protein transferase ZNF451, E3 SUMO-protein ligase ZNF451

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF451 Polyclonal antibody (25228-1-AP)

    WB analysis of HeLa using 25228-1-AP

    WB result of ZNF451 antibody (25228-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF451 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using ZNF451 Polyclonal antibody (25228-1-AP)

    WB analysis of HeLa using 25228-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25228-1-AP (ZNF451 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZNF451 Polyclonal antibody (25228-1-AP)

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZNF451 Polyclonal antibody (25228-1-AP)

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using ZNF451 Polyclonal antibody (25228-1-AP)

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ZNF451 Polyclonal antibody (25228-1-AP)

    IHC staining of human placenta using 25228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using ZNF451 Polyclonal antibody (25228-1-AP)

    IHC staining of human placenta using 25228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF451 Polyclonal antibody (25228-1-AP)

    IF Staining of HeLa using 25228-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25228-1-AP (ZNF451 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25228-1-AP targets ZNF451 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF451 fusion protein Ag18346
Full Name zinc finger protein 451
Calculated Molecular Weight 1061 aa, 121 kDa
Observed Molecular Weight 121 kDa
GenBank Accession NumberBC021712
Gene Symbol ZNF451
Gene ID (NCBI) 26036
RRIDAB_2879975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF451 antibody 25228-1-APDownload protocol
IHC protocol for ZNF451 antibody 25228-1-APDownload protocol
IF protocol for ZNF451 antibody 25228-1-APDownload protocol
IP protocol for ZNF451 antibody 25228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1

Authors - Carlos F De La Cruz-Herrera
View Article
humanWB,IHC,IF

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
  • KD Validated
View Article
mouseWB

Respir Res

Loss of ZNF451 mediates fibroblast activation and promotes lung fibrosis

Authors - Hong Peng
View Article