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ZNF446 Polyclonal antibody

ZNF446 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16218-1-AP

Synonyms

ZSCAN30, ZKSCAN20

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of SH-SY5Y cells using ZNF446 Polyclonal antibody (16218-1-AP)

    WB analysis of SH-SY5Y using 16218-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16218-1-AP (ZNF446 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using ZNF446 Polyclonal antibody (16218-1-AP)

    WB analysis of SH-SY5Y using 16218-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16218-1-AP (ZNF446 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using ZNF446 Polyclonal antibody (16218-1-AP)

    IF Staining of SH-SY5Y using 16218-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 16218-1-AP (ZNF446 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16218-1-AP targets ZNF446 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF446 fusion protein Ag9266
Full Name zinc finger protein 446
Calculated Molecular Weight 422 aa, 46 kDa
Observed Molecular Weight 49-55 kDa
GenBank Accession NumberBC017206
Gene Symbol ZNF446
Gene ID (NCBI) 55663
RRIDAB_2878231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF446 antibody 16218-1-APDownload protocol
IF protocol for ZNF446 antibody 16218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.

Authors - Zane A Gibbs
View Article