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ZNF397 Polyclonal antibody

ZNF397 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20799-1-AP

Synonyms

zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ZNF397 Polyclonal antibody (20799-1-AP)

    WB analysis of PC-3 using 20799-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ZNF397 Polyclonal antibody (20799-1-AP)

    WB analysis of PC-3 using 20799-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using ZNF397 Polyclonal antibody (20799-1-AP)

    WB analysis of mouse testis using 20799-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ZNF397 Polyclonal antibody (20799-1-AP)

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ZNF397 Polyclonal antibody (20799-1-AP)

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-3 cells, mouse testis tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20799-1-AP targets ZNF397 in WB, IHC, ELISA applications and shows reactivity with human, mouse, Rat samples.

Tested Reactivity human, mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF397 fusion protein Ag14712
Full Name zinc finger protein 397
Calculated Molecular Weight 534 aa, 61 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC006172
Gene Symbol ZNF397
Gene ID (NCBI) 84307
RRIDAB_2878741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF397 (Zinc Finger Protein 397) is conserved in placental mammals. ZNF397 as a bona fide coactivator of the androgen receptor (AR), essential for the transcriptional program governing AR-driven luminal lineage.

Protocols

Product Specific Protocols
WB protocol for ZNF397 antibody 20799-1-APDownload protocol
IHC protocol for ZNF397 antibody 20799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols