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ZNF394 Polyclonal antibody

ZNF394 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25785-1-AP

Synonyms

zinc finger protein 394, ZKSCAN14, ZNF394

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ZNF394 Polyclonal antibody (25785-1-AP)

    WB analysis of mouse brain using 25785-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using ZNF394 Polyclonal antibody (25785-1-AP)

    WB analysis of fetal human brain using 25785-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ZNF394 Polyclonal antibody (25785-1-AP)

    WB analysis of mouse spleen using 25785-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF394 Polyclonal antibody (25785-1-AP)

    WB analysis of HepG2 using 25785-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25785-1-AP (ZNF394 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, fetal human brain tissue, HepG2 cells, mouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25785-1-AP is ZNF394 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF394 fusion protein Ag22655
Full Name zinc finger protein 394
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC051847
Gene Symbol ZNF394
Gene ID (NCBI) 84124
RRIDAB_2880239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF394 antibody 25785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Simone (Verified Customer) (04-25-2022)

From 6 well plate pellet, lysed with 1ml NP40 buffer

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T cells