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ZNF354A Polyclonal antibody

ZNF354A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14072-1-AP

Synonyms

EZNF, HKL1, KID 1, KID1, TCF 17, TCF17, Transcription factor 17, zinc finger protein 354A, Zinc finger protein eZNF, ZNF354A

Validation Data Gallery

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  • Western Blot (WB) analysis of DU 145 cells using ZNF354A Polyclonal antibody (14072-1-AP)

    WB analysis of DU 145 using 14072-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 14072-1-AP (ZNF354A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inDU 145 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14072-1-AP targets ZNF354A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF354A fusion protein Ag5142
Full Name zinc finger protein 354A
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC047105
Gene Symbol ZNF354A
Gene ID (NCBI) 6940
RRIDAB_2217732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF354A, also called EZNF, KID-1 or TCF17, belongs to the Kruppel C2H2-type zinc-finger family of proteins that contain KRAB domains and act as transcriptional regulators. Expressed primarily in the adult kidney, ZNF354A is a transcriptional repressor that plays a role in late renal development and is suppressed after renal ischemia. The N-terminus of ZNF354A contains the KRAB domain which confers transcriptional repressor activity, while the C-terminus contains multiple Cys2His2-zinc fingers. ZNF354A is located in the nucleolus and is thought to specifically influence development of the proximal tubule by shutting off dispensable or inhibitory genes. Reduced ZNF354A expression prevents proper cell differentiation and may, therefore, be implicated in renal carcinoma.

Protocols

Product Specific Protocols
WB protocol for ZNF354A antibody 14072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols