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ZNF277 Polyclonal antibody

ZNF277 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25094-1-AP

Synonyms

NRIF4, zinc finger protein 277, ZNF277, ZNF277P

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using ZNF277 Polyclonal antibody (25094-1-AP)

    WB analysis of PC-3 using 25094-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ZNF277 Polyclonal antibody (25094-1-AP)

    WB analysis of Jurkat using 25094-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF277 Polyclonal antibody (25094-1-AP)

    IF Staining of HepG2 using 25094-1-AP

    Immunofluorescent analysis of HepG2 cells using 25094-1-AP (ZNF277 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inPC-3 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25094-1-AP is ZNF277 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF277 fusion protein Ag18979
Full Name zinc finger protein 277
Calculated Molecular Weight 450 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC020626
Gene Symbol ZNF277
Gene ID (NCBI) 11179
RRIDAB_2879893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF277 antibody 25094-1-APDownload protocol
IF protocol for ZNF277 antibody 25094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols