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ZNF266 Polyclonal antibody

ZNF266 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11374-1-AP

Synonyms

HZF1, KIAA2007, zinc finger protein 266, Zinc finger protein HZF1, ZNF266

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ZNF266 Polyclonal antibody (11374-1-AP)

    WB analysis of HeLa using 11374-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11374-1-AP (ZNF266 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ZNF266 Polyclonal antibody (11374-1-AP)

    IHC staining of human prostate cancer using 11374-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11374-1-AP (ZNF266 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11374-1-AP targets ZNF266 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF266 fusion protein Ag1933
Full Name zinc finger protein 266
Calculated Molecular Weight 670 aa, 70 kDa
Observed Molecular Weight 50~55+70~75 kDa
GenBank Accession NumberBC017407
Gene Symbol ZNF266
Gene ID (NCBI) 10781
RRIDAB_2257588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF266, also named as HZF1, was expressed in ubiquitous tissues and various hematopoietic cell lines. Expression of ZNF266 in K562 cells was increased after being induced by hemin or phorbol myristate acetate (PMA), suggesting that HZF1 play important roles in erythroid and megakaryocytic differentiation.

Protocols

Product Specific Protocols
WB protocol for ZNF266 antibody 11374-1-APDownload protocol
IHC protocol for ZNF266 antibody 11374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols