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ZNF259 Polyclonal antibody

ZNF259 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15558-1-AP

Synonyms

ZPR1

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using ZNF259 Polyclonal antibody (15558-1-AP)

    WB analysis of human brain using 15558-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ZNF259 Polyclonal antibody (15558-1-AP)

    WB analysis of HepG2 using 15558-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using ZNF259 Polyclonal antibody (15558-1-AP)

    WB analysis of DU 145 using 15558-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF259 Polyclonal antibody (15558-1-AP)

    WB analysis of HeLa using 15558-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15558-1-AP (ZNF259 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using ZNF259 Polyclonal antibody (15558-1-AP)

    IHC staining of human skin using 15558-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15558-1-AP (ZNF259 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ZNF259 Polyclonal antibody (15558-1-AP)

    IHC staining of human ovary cancer using 15558-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 15558-1-AP (ZNF259 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin tissue using ZNF259 Polyclonal antibody (15558-1-AP)

    IHC staining of human skin using 15558-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 15558-1-AP (ZNF259 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF259 Polyclonal antibody (15558-1-AP)

    IF Staining of HepG2 using 15558-1-AP

    Immunofluorescent analysis of HepG2 cells using 15558-1-AP (ZNF259 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman brain tissue, DU 145 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15558-1-AP targets ZNF259 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF259 fusion protein Ag7980
Full Name zinc finger protein 259
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC004256
Gene Symbol ZNF259
Gene ID (NCBI) 8882
RRIDAB_10858477
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF259, also named as Zpr1, is an evolutionarily conserved protein characterized by two C4 zinc fingers and two Zpr1-specific conserved homology domains. Zpr1 was first identified in mammalian cells as a cytoplasmic protein that was capable of binding the intracellular domain of the unactivated EGF receptor (EGFR). The MW of this protein is 51 kDa, and this antibody specially recognises the 51 kDa protein.

Protocols

Product Specific Protocols
WB protocol for ZNF259 antibody 15558-1-APDownload protocol
IHC protocol for ZNF259 antibody 15558-1-APDownload protocol
IF protocol for ZNF259 antibody 15558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols