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ZNF256 Polyclonal antibody

ZNF256 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 10360-1-AP

Synonyms

BMZF 3, BMZF3, Bone marrow zinc finger 3, zinc finger protein 256, ZNF256

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ZNF256 Polyclonal antibody (10360-1-AP)

    WB analysis of mouse testis using 10360-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10360-1-AP (ZNF256 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using ZNF256 Polyclonal antibody (10360-1-AP)

    WB analysis of HL-60 using 10360-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10360-1-AP (ZNF256 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse testis tissue, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10360-1-AP targets ZNF256 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF256 fusion protein Ag0390
Full Name zinc finger protein 256
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC001438
Gene Symbol ZNF256
Gene ID (NCBI) 10172
RRIDAB_2219240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZNF256 as a transcriptional repressor that plays a role in cell proliferation. ZNF256 localizes in the nucleus and contains the KRAB domain that mediates interaction with TRIM28 and is required for transcriptional repressor activity. ZNF256 exists as two isoforms and the calculated molecular weight of isoforms is 55 and 75 kDa.

Protocols

Product Specific Protocols
WB protocol for ZNF256 antibody 10360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols