• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ZNF24 Polyclonal antibody

ZNF24 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11219-1-AP

Synonyms

Zinc finger protein 191, Zinc finger and SCAN domain-containing protein 3, Zfp191, RSG-A, RSG A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using ZNF24 Polyclonal antibody (11219-1-AP)

    WB analysis of HeLa using 11219-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using ZNF24 Polyclonal antibody (11219-1-AP)

    WB analysis of mouse liver using 11219-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF24 Polyclonal antibody (11219-1-AP)

    WB analysis of HeLa using 11219-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11219-1-AP (ZNF24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ZNF24 Polyclonal antibody (11219-1-AP)

    IHC staining of human ovary tumor using 11219-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ZNF24 Polyclonal antibody (11219-1-AP)

    IHC staining of human ovary tumor using 11219-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF24 Polyclonal antibody (11219-1-AP)

    IHC staining of human testis using 11219-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZNF24 Polyclonal antibody (11219-1-AP)

    IHC staining of human testis using 11219-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ZNF24 Polyclonal antibody (11219-1-AP)

    IHC staining of human breast cancer using 11219-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ZNF24 Polyclonal antibody (11219-1-AP)

    IHC staining of human breast cancer using 11219-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ZNF24 Polyclonal antibody (11219-1-AP)

    IF Staining of HeLa using 11219-1-AP

    Immunofluorescent analysis of HeLa cells using 11219-1-AP (ZNF24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue
Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11219-1-AP targets ZNF24 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF24 fusion protein Ag1724
Full Name zinc finger protein 24
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC016801
Gene Symbol ZNF24
Gene ID (NCBI) 7572
RRIDAB_10888626
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger protein 24 (ZNF24; also known as KOX17, Zfp191 or ZNF191) is a member of the Kruppel-like zinc finger (ZF) transcription factor family, which plays a critical role in cell proliferation and differentiation. There're two isoforms with calculated MW 42KDa and 22KDa. ZNF24 protein can be modificated with SUMO.

Protocols

Product Specific Protocols
WB protocol for ZNF24 antibody 11219-1-APDownload protocol
IHC protocol for ZNF24 antibody 11219-1-APDownload protocol
IF protocol for ZNF24 antibody 11219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

LncRNA ZNFTR functions as an inhibitor in pancreatic cancer by modulating ATF3/ZNF24/VEGFA pathway.

Authors - Wei Li
  • KD Validated
View Article
humanIHC,WB,IF

Front Oncol

ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation

Authors - Daqi Jia
View Article
humanIHC

Exp Cell Res

Tumor-suppressive zinc finger protein 24 (ZNF24) sensitizes colorectal cancer cells to 5-fluorouracil by inhibiting the Wnt pathway and activating the p53 signaling

Authors - Fanqi Meng
View Article