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ZNF23 Polyclonal antibody

ZNF23 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21322-1-AP

Synonyms

Zinc finger protein KOX16, Zinc finger protein 612, Zinc finger protein 359, Zinc finger protein 23, Zfp612

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of HepG2 using 21322-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of mouse brain using 21322-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of mouse lung using 21322-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of mouse spleen using 21322-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of A2780 using 21322-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SKOV-3 cells using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of SKOV-3 using 21322-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of HeLa using 21322-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using ZNF23 Polyclonal antibody (21322-1-AP)

    WB analysis of A2780 using 21322-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ZNF23 Polyclonal antibody (21322-1-AP)

    IP experiment of mouse brain using 21322-1-AP

    IP result of anti-ZNF23 (IP:21322-1-AP, 4ug; Detection:21322-1-AP 1:800) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ZNF23 Polyclonal antibody (21322-1-AP)

    IF Staining of HepG2 using 21322-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF23 antibody 21322-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHepG2 cells, A2780 cells, HeLa cells, mouse brain tissue, mouse lung tissue, mouse spleen tissue, SKOV-3 cells
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21322-1-AP targets ZNF23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF23 fusion protein Ag14755
Full Name zinc finger protein 23 (KOX 16)
Calculated Molecular Weight 643 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC007256
Gene Symbol ZNF23
Gene ID (NCBI) 7571
RRIDAB_2878839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF23 antibody 21322-1-APDownload protocol
IF protocol for ZNF23 antibody 21322-1-APDownload protocol
IP protocol for ZNF23 antibody 21322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Physiol Biochem

ZNF23 Suppresses Cutaneous Melanoma Cell Malignancy via Mitochondria-Dependent Pathway.

Authors - Xin Zhang
View Article