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ZNF146 Polyclonal antibody

ZNF146 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 20385-1-AP

Synonyms

Only zinc finger protein, OZF, zinc finger protein 146, Zinc finger protein OZF, ZNF146

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using ZNF146 Polyclonal antibody (20385-1-AP)

    WB analysis of mouse kidney using 20385-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using ZNF146 Polyclonal antibody (20385-1-AP)

    WB analysis of mouse lung using 20385-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using ZNF146 Polyclonal antibody (20385-1-AP)

    IHC staining of human heart using 20385-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ZNF146 Polyclonal antibody (20385-1-AP)

    IHC staining of human heart using 20385-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20385-1-AP is ZNF146 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZNF146 fusion protein Ag14223
Full Name zinc finger protein 146
Calculated Molecular Weight 292 aa, 33 kDa
Observed Molecular Weight 20-25 kDa, 33-35 kDa
GenBank Accession NumberBC005154
Gene Symbol ZNF146
Gene ID (NCBI) 7705
RRIDAB_10694154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZNF146 antibody 20385-1-APDownload protocol
IHC protocol for ZNF146 antibody 20385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanChIP

Reproduction

ZNF146 regulates cell cycle progression via TFDP1 and DEPDC1B in ovarian cancer cells

Authors - Ruixue Zhao
View Article
human,mouseWB,IHC

J Gastrointest Oncol

Hsa_circ_NOTCH3 regulates ZNF146 through sponge adsorption of miR-875-5p to promote tumorigenesis of hepatocellular carcinoma.

Authors - Lei Bao
View Article