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ZMYND12 Polyclonal antibody

ZMYND12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 25587-1-AP

Synonyms

ZMYND12

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using ZMYND12 Polyclonal antibody (25587-1-AP)

    WB analysis of mouse testis using 25587-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25587-1-AP (ZMYND12 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using ZMYND12 Polyclonal antibody (25587-1-AP)

    WB analysis of rat testis using 25587-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 25587-1-AP (ZMYND12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using ZMYND12 Polyclonal antibody (25587-1-AP)

    IHC staining of human testis using 25587-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25587-1-AP (ZMYND12 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using ZMYND12 Polyclonal antibody (25587-1-AP)

    IHC staining of human testis using 25587-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25587-1-AP (ZMYND12 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25587-1-AP is ZMYND12 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZMYND12 fusion protein Ag22358
Full Name zinc finger, MYND-type containing 12
Calculated Molecular Weight 365 aa, 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC024186
Gene Symbol ZMYND12
Gene ID (NCBI) 84217
RRIDAB_2880142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZMYND12 antibody 25587-1-APDownload protocol
IHC protocol for ZMYND12 antibody 25587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP,IF

Cell Mol Life Sci

ZMYND12 serves as an IDAd subunit that is essential for sperm motility in mice

Authors - Chang Wang
  • KO Validated
View Article