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  • Featured Product
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ZKSCAN1 Polyclonal antibody

ZKSCAN1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25214-1-AP

Synonyms

9130423L19Rik, KOX18, PHZ 37, Zinc finger protein 139, Zinc finger protein 36, Zinc finger protein KOX18, ZKSCAN1, ZNF139, ZNF36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ZKSCAN1 Polyclonal antibody (25214-1-AP)

    WB analysis using 25214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25214-1-AP (ZKSCAN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ZKSCAN1 Polyclonal antibody (25214-1-AP)

    WB analysis of HEK-293 using 25214-1-AP

    WB result of ZKSCAN1 antibody (25214-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZKSCAN1 transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ZKSCAN1 Polyclonal antibody (25214-1-AP)

    IF Staining of A431 using 25214-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZKSCAN1 antibody (25214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25214-1-AP targets ZKSCAN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZKSCAN1 fusion protein Ag19721
Full Name zinc finger with KRAB and SCAN domains 1
Calculated Molecular Weight 563 aa, 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC022378
Gene Symbol ZKSCAN1
Gene ID (NCBI) 7586
RRIDAB_2879963
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZKSCAN1(Zinc finger protein with KRAB and SCAN domains 1), also named as ZNF139, is a member in transcription factor zinc finger protein family(PMID: 24515389). Zinc finger protein 139 (ZNF139) gene is proved play an important role in gastric cancer, overexpression of ZNF139 correlated with tumor differentiation, invasion depth, clinical stage, lymphatic metastasis, and blood vessel invasion(PMID: 25436386, PMID: 25561801). The molecular weight of ZKSCAN1 is 64 kDa.

Protocols

Product Specific Protocols
WB protocol for ZKSCAN1 antibody 25214-1-APDownload protocol
IF protocol for ZKSCAN1 antibody 25214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer

Authors - Bei-Bei Sun
View Article