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ZDHHC8 Polyclonal antibody

ZDHHC8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27179-1-AP

Synonyms

DHHC 8, KIAA1292, ZDHHC8, ZDHHCL1, Zinc finger protein 378, ZNF378

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ZDHHC8 Polyclonal antibody (27179-1-AP)

    WB analysis using 27179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27179-1-AP (ZDHHC8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using ZDHHC8 Polyclonal antibody (27179-1-AP)

    WB analysis of K-562 using 27179-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27179-1-AP (ZDHHC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, K-562 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27179-1-AP targets ZDHHC8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZDHHC8 fusion protein Ag26028
Full Name zinc finger, DHHC-type containing 8
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC053544
Gene Symbol ZDHHC8
Gene ID (NCBI) 29801
RRIDAB_2880789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ZDHHC8 antibody 27179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cancer Lett

AMPKα1-mediated ZDHHC8 phosphorylation promotes the palmitoylation of SLC7A11 to facilitate ferroptosis resistance in glioblastoma

Authors - Zhangjie Wang
View Article
humanWB

Sci Rep

Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages

Authors - Ryosuke Shimai
View Article